About : Protocol Exchange. Protocol Exchange. The Protocol Exchange is an Open Repository for the deposition and sharing of protocols for scientific research. These protocols are posted directly on the Protocol Exchange by their authors and hence have not been further styled, peer reviewed or copy edited.
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Rather they are made freely available to the scientific community for use and comment. Mission and Scope. The Protocol Exchange strives to facilitate rapid and unencumbered distribution of protocols for scientific research. We welcome Protocols from any branch of science however we focus particularly on Protocols being used to answer outstanding biological and biomedical science research questions, which includes methods grounded in physics and chemistry with a practical application to the study of biological problems.
The content of Protocol Exchange is currently classified under the following broad subject categories: Analytical chemistry; Biochemistry; Cell biology; Cell culture; Chemical modification; Computational biology; Developmental biology; Epigenomics; Genetic analysis; Genetic modification; Genomics; Imaging; Immunological techniques; Isolation, Purification and Separation; Lipidomics; Materials science; Mathematics and computing; Metabolomics; Microbiology; Model organisms; Nanotechnology; Neuroscience; Nucleic acid based molecular biology; Optics and photonics; Pharmacology; Physics; Plant biology; Protein analysis; Proteomics; Spectroscopy; Structural biology; Synthetic chemistry; Tissue culture; Toxicology; and Virology. If your protocol does not fall into any of these categories please contact us at protocol. Copyright. Protocols on the Exchange are presented subject to a Creative Commons Attribution- Non. Commercial licence. This means that anyone is free to read, copy, distribute, transmit and adapt the protocols provided that attribution of the work is always given by its proper citation and that such reuse is not for commercial purposes.
Citing protocols from the Protocol Exchange. If you are adding the citation to a reference list, the Journal Name is “Protocol Exchange”, and the instead of a volume and page number you would add the DOI. You might arrange the information as shown in the example below: Kanoativ, M, Javeherian, S and Krylov, S. Aptamer- facilitated Protein Isolation from Cells” Protocol Exchange (2. DOI: 1. 0. 1. 03. If you want to insert a link to the protocol either in a paper or on a webpage, please use the DOI rather than the current webaddress. Laboratory Groups.
Protocols Exchange is organised around the concept of Laboratory Groups. All Protocols are associated with a Lab Group and authors cannot share protocols except as members of a Lab Group. Every Lab Group has an individual page on which is listed all their Protocols within the Protocol Exchange. There is also a Publications, list which can be used to link to papers from the group in which the protocols are used, publications by the group in the peer- reviewed journal Nature Protocols, the most significant research papers produced by the lab groups, and so on. Members of a lab group can also see drafts of Protocols that are being prepared for sharing through Protocol Exchange. Joining a Lab Group.
The first time you come to share a protocol, you will be re- directed to a page where you are prompted to either Create a Lab Group or to Join an existing one. Lab Groups require a name and an owner who controls the settings and membership of the group. Although not required we also suggest that you include the physical address of the lab and a link to the external home page of the Lab if one exists. There are three possible settings for a Lab Group: 1. Open – This means that any user of Protocol Exchange can join this group. This is done using the ‘Join Group’ button on these Lab Group’s pages or in the lab Group listing.
Accepting Requests – This means that any user of Protocol Exchange can request to join this Lab Group, this application will be accepted or declined by the Lab Group Owner. This is done using the ‘Apply to Group’ button on these Lab Group’s pages or in the lab Group listing. Invitation Only – This means that you can only become a member of the Lab Group if invited by the Lab Group Owner. We recommend that when setting up a Lab Group you set it to ‘Invitation Only’, at least initially, unless you have a specific desire to create a more open group. All details and settings of any Lab Group can be changed by the Lab Group Owner at any time. Lab Group Owners can also remove members from the group.
Choosing a name for your Lab Group. We recommend that the names of Lab Groups be short and descriptive. A typical format would follow this example: Ben Davis Lab (Oxford). The maximum length is 2. In addition to the name Lab Groups can also choose to display their address and a URL for their institutional home page.
Uploading a logo. A personalised log for the Lab Group can also be used. The maximum allowed dimensions for this logo are 2. If no image is supplied then a stock image of 1.
Academic/Supplier Groups. Lab Groups can be classified as Academic if they are associated with a University or similar institution, or Commercial if they are associated with a private company. Protocols originating from an Academic Lab Group are labelled as Community Contributed; protocols from Commercial Lab Groups are labelled as Supplier Contributed. Your activity. On the Protocol Exchange site, you can Share Protocols; Create, Manage and Join Lab Groups; and make a list of your Favourite Protocols from the Protocol Exchange and Nature Protocols.
Your Activity can be accessed from the right hand navigation. The Your Activity screen displays: Any invitations to join Lab Groups that you have received.
These can be accepted or declined by pressing the ‘Join’ or ‘Decline’ buttons. Any applications to join Lab Groups that are outstanding. These can be cancelled if you no longer wish to join this group. Any applications that have been made to join the Lab Groups for which you are the Owner. These can be accepted or declined by pressing the ‘Accept’ or ‘Decline’ buttons. Also within the Your Activity box you have access to Your Protocols. This lists all the Protocols that you have shared through the Protocol Exchange.
Those that are still in draft form can be Submitted for editorial moderation and sharing, Edited further or Discarded. Those protocols that have been submitted to moderation but not yet formally shared can be further Edited. Your Lab Groups. This lists the Lab Groups for which you are the Owner and also those for which you are a member. You can also create new Lab Groups and apply to join any Lab Group that entertains membership requests.
Your Favourites. This lists the Protocols that you have identified as of particular interest to you. It is also possible to create RSS feeds for Subject Terms and Keywords selected from the Browse page.
This is done by inserting . URL of the results page. For example if you chose the Subject Term “Biochemistry”, the rss feed would be: http: //www. Bfacets%5. D%5. Bcategory%5. D=Biochemistry Your preferences.
From here you can edit your information on Protocol Exchange: Email address, Title, First and Last name, and Country. You can also change your Password through this screen. There is a tick box called “Accepting invitations”.
Click this box if you would like owners of Lab Groups to be able to invite you to their groups. Format. We ask that when preparing Protocols to be shared through the Protocol Exchange authors use a slightly simplified version of the format used on Nature Protocols. For details of Nature Protocols format please see their Guide to Authors. In brief we ask that the Protocol is presented in the following sections: Protocol Title (this is a required field)Titles should be distinctive and certainly different from and Associated Publications. Italics, Superscript and Subscript can be used. Authors. There is no limit to the number of authors on a Protocol. A first and last name is required for all authors.
Complete author details (including Affiliation and Email) are required for all Corresponding Authors. At least one author must be designated as the Corresponding Author in order to submit the protocol.
The Corresponding Author will usually be the person uploading the Protocol but this is not an absolute requirement. Laboratory Group (this is a required field)All Protocols must be assigned to one of the Laboratory Groups of which the author is a member. Subject Terms (this is a required field)All Protocols must be assigned at least one of our subject terms. If your Protocol does not fit into any of our categories please contact us on Protocol. Exchange@nature. com. Keywords (this is a required field)Enter as many keywords or keyphrases as you feel are appropriate for your Protocol.
The list should be separated by commas. Formatting is restricted to super- and subscript. Please avoid these where possible e. I. Abstract. The Abstract should give a brief description of the Protocol, its applicability, strengths, weaknesses, and the time taken for it to be completed .
Consider limiting this text to about 1. Introduction. The Introduction allows for a broader discussion of the Protocol and the basic considerations needed before embarking on its use. The Introduction also gives you an opportunity to set the Protocol in context and discuss related or alternative protocols. You might want to include a Flow Diagram here. You can upload images by going to “Images and attachments”, and follow the instructions from “How do I enter links, images and formatted text” Reagents and Equipment.
A full list of reagents and equipment needed for the protocol should be supplied with manufacturer’s catalogue numbers wherever possible. Procedure (this is a required field)This should be formatted as a list of numbered steps each containing at least one sentence in the active tense e.